The couple, who have been married for 15 years and have children Jaden and Willow together, are said to be living separate lives and have not been pictured together since August.
"They were considered to have one of the most solid marriages in Hollywood, but lately it hasn't seemed that way," an insider told The Mirror. "Things seemed tense for months."
Will and Jada have been hit by marital rumours for months
Jada herself has also reportedly suggested that the couple's marriage was on the rocks.
"When you are going through a storm with your spouse, stop thinking about what you 'believe' a husband or wife should be," said Jada, according to a fellow audience member at a charity event.
Reports have also surfaced that Will and Jada are not living under the same roof, and have put their family home up for sale.
While the After Earth actor is in New Orleans filming his latest movie Focus, Jada is believed to have moved into her brother Caleeb's home in Los Angeles.
Jaden, Willow, Will, Jada and Trey attend the After Earth premiere in New York
This is not the first time the couple have been hit by rumours of marital problems. In April the Hawthorne actress said Will could do "whatever he wants" in their marriage, but later added in a post on Facebook that this did not mean they had an open relationship.
Will has another son, 20-year-old Trey, from his previous marriage to actress Sheree Fletcher.