Viewers of the hour-long BBC coverage of Dolly's set on Sunday aired their criticism over social media site Twitter, with Sky News presenter Kay Burley one of the first to tweet:
Oh, Dolly is miming. How disappointing— Kay Burley (@KayBurley) June 29, 2014
I think I know quite a lot about how TV is made. Why are people saying @DollyParton is miming? She’s fooling me…— Stephen Fry (@stephenfry) June 29, 2014
Bon Jovi's Richie Sambora joined Dolly Parton on stage
Dolly's performance reportedly drew the biggest crowd of the 2014 festival, matching the figures of last year's Rolling Stones set.
"It is such an honour and such a thrill to be at the Glastonbury Festival, I've been waiting a lifetime for this", Dolly told the crowd.
DOLLY PARTON 'INCREDIBLY EXCITED' FOR GLASTONBURY DEBUT
"We're glad that you came to see me!" she added before being joined by the crowd in singing her famous song Jolene.
Dolly Parton's Glastonbury Festival performance was one of the year's most watched
When asked about the criticism, her spokesperson told The Sunday Mirror: "No, she sings live. Some people don't know an amazing singer when they hear one", however in the past Dolly has admitted that she often uses pre-recorded elements to her shows.
"Yes, there are portions of the songs in the show that are pre-recorded.
"I tell you that at the beginning of the show when I say, 'If you notice some enhancement or doctoring in the show, it's not to fool or trick anyone. It's an effort to entertain by being able to have a bigger production and, hopefully, a better sound," she told fan website Dollymania.