Mr Ryan reportedly claimed that the Football League wanted "cleared funds of £5M from day one", but he told BBC Radio Sheffield: "I told them I had assets well above that figure and it's just been disregarded."
Louis Tomlinson is a big football fan
He added: "As far as I am concerned this is a dark day for football. Louis and I were just looking to do the right thing but this is the end of my involvement in football now.
It has been a long love affair between myself and Doncaster Rovers but it has hit the buffers today. The way I feel right now I doubt I will ever go to another football match in my life."
Louis and John, who have tried twice before to finalise the deal, chose to save their club after it was relegated to the third tier of English football in May.
Louis Tomlinson and John Ryan in their crowdfunder video
"For me, this is all about the football, the community and restoring the excitement and desire to making Doncaster Rovers the most exciting club to play for in Yorkshire," wrote Louis in a statement.
"I want to see the Doncaster Rovers supporters get the club the success it deserves. I grew up in Doncaster and have felt the love for football run through the town, it's for that reason that I have a real personal passion to make Doncaster Rovers a success story.
"This is a big step which I believe will open up opportunities to provide a very, very exciting future for the club and its supporters."