The 28-year-old Prince, who is Patron of the charity, will join the team inside special environmental test chambers which simulate the extreme conditions they will face in Antarctica.
He will acclimatise with the team, known as Team Glenfiddich, test equipment and practice the routine for the expedition in extreme conditions, including skiing and setting up camp.
The test comes two months ahead of a race to the South Pole, when Harry will join three teams of wounded servicemen and women in November and December.
Harry and his teammates, who all have either physical or cognitive injuries sustained in the line of duty, will cover more than 200 miles in total.
They face treks of around 10 miles per day through temperatures as low as -45°C and battling 50mph winds while pulling heavy sledges.
The new uncle has already been in training for the tough challenge, donning skis to cross the Langjokull Glacier in Iceland earlier this month, accompanied by actor Dominic West, who will also head off to the South Pole in November.
In the final leg of preparation, the team will practice traversing the harsh terrain of Antarctica in the Cold Chamber on 17 September, using cross trainers to simulate skiing for two hours at a time before taking a ten-minute break and repeating the activity for the following 12 hours.
They will then set up camp for the night, a routine vital to their survival, and one which will take them several hours a day.
The expedition aims to highlight the extraordinary courage and determination of the men and women who have been wounded while serving our countries.
Prince Harry was also Patron of the Walking with the Wounded trek to the North Pole in 2011 and the Everest Expedition in 2012.