A judge has decided King Juan Carlos' youngest daughter should appear in court on 8 March at 10am as part of the case investigating her husband, Iñaki Urdangarin, who is accused of embezzlement.
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Palma de Mallorca court judge Jose Castro has ordered Cristina to answer questions about her business partnership with her husband in a firm called Aizoon.
Cristina's husband is suspected of having overcharged local authorities for organising sporting and tourism events.
She will be asked about spending in the company's accounts over the period 2006-2008.
The Spanish royal's lawyer has said he will appeal against the court summons, and has evidence to prove the innocence of his client.
Last year, Cristina was ordered to appear in court as part of the investigation, but the decision was overturned after the regional court of Mallorca, which is managing the case, found insufficient evidence to link her to her husband's business affairs.
As news of the summons broke, it was business as usual for the Spanish royal, who was seen taking her children to school in Geneva, where the family is based. Later she went to her office as normal.