Matt Baker is mourning pet dog Meg who became familiar to viewers through appearances in Blue Peter, Countryfile and Crufts coverage.
Meg, who was 11 years old, even made an appearance on Radio 4's The Archers and was seen on screen when the One Show presenter took part in Strictly Come Dancing last year.
Blue Peter bosses have also announced the death of another former show pet, Oke the cat, who had a 13-year stint on the programme.
It brings the number of animal stars of the show to have died so far this year to four.
Border collie Meg appeared on the show alongside Matt for five years. She was the eighth dog on the BBC1 series and appeared with other show dogs Mabel and Lucy.
The presenter had been brought up on a farm and was keen to have his own pet on screen.
She first joined in February 2001 as a nine-week old pup and also appeared with six other presenters during her time on the children's show.
Assignments included a barking part in The Archers, competing in a sheep dog trial and passing a police sniffer dog challenge.
After leaving the show when Matt moved on, she joined him on his later shows such as Crufts and Countryfile.
He said: "I felt as soon as we met she was something special, and it wasn't just her recognisable triangular ears. I never dreamt she would turn out to be my most faithful friend.
"Not only did she bring happiness to millions of children and dog lovers as a brilliant Blue Peter dog, always getting far more fan mail than me, she was an action hero, full of fun and always had play at heart."
Photo: © BBC