All eyes were on rumoured couple Kristen Stewart and Robert Pattinson, who were careful not to be photographed together all night.
Instead Kristen posed on the red carpet with co-star Taylor Lautner and told reporters the night should be a celebration of his work.
"I'm so proud of that kid," said the 19-year-old actress, who eschewed her usual rock chick look for an elegant Oscar de la Renta gown.
"He's taught me more than I've ever been able to bestow on him. I think he's the most centred of everybody here."
The biggest screams of the night from fans – many of whom had camped overnight to be sure of getting a glimpse of their idols – were reserved for Robert, dapper in a smart suit.
Also at the premiere of the film – which opens in the UK and US this Friday – was Dakota Fanning. The War Of The Worlds actress, who plays evil vampire Jane in the film, looked all grown-up in a lacy black dress by Valentino.
Meanwhile British actor Michael Sheen was accompanied by his excited daughter Lily, who is a massive fan of the Twilight books.
It was Michael's ten-year-old daughter with Kate Beckinsale who inspired him to take the part of Volturi vampire clan leader Aro in the big-screen version of the second book.
Guests at the world premiere included Birmingham-born TV presenter Cat Deeley, who attended with her actor boyfriend Jack Huston.